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KMID : 0380020100250040363
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 4 p.363 ~ p.370
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Extraction, Purification and Property of the lipid from Scenedesmus sp.
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Kim Na-Young
Oh Sung-Ho
Choi Woon-Yong
Lee Hyeon-Yong
Lee Shin-Young
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Abstract
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Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was 21.38 ± 1.03 wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of C16∼C22 with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.
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KEYWORD
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Scenedesmus sp., lipid, solvent extract, fractionation, property
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